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Physical interactions between Skp1 and calcyclin binding protein/Siah-1 in SGC7901 cells. Nuclear protein extracts from gastric cancer cells treated with 10-8 mol/L gastrin were immunoprecipitated with calcyclin binding protein/Siah-1 (CacyBP/SIP) MAb or control IgG. Immune complexes were analyzed by immunoblotting using an anti-Skp1 antibody with ECL-based detection.
