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. 2016 Apr 21;22(15):3992–4001. doi: 10.3748/wjg.v22.i15.3992

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©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.

PMC Copyright notice

Interaction between Skp1 and calcyclin binding protein/Siah-1 (1-72) in SGC7901-calcyclin binding protein/Siah-1si transfected with pEGFP/C1-calcyclin binding protein/Siah-1 (1-72). Nuclear protein extracts from cells transfected with pEGFP/C1-CacyBP/SIP or pEGFP/C1- CacyBP/SIP (1-72) after treatment with 10-8 mol/L gastrin were immunoprecipitated with anti-GFP antibody or control IgG. Immune complexes were analyzed by immunoblotting using an anti-Skp1/GFP antibody with ECL-based detection. CacyBP/SIP: Calcyclin binding protein/Siah-1 interacting protein.