Fig. 3.

Histone 3 acetylation (AcH3) on the microgrooved substrate. (A) Western blot showing the AcH3 levels on the microgrooved versus flat substrate. (B) Representative immunostaining for AcH3 (scale bar: 20 μm). (C) Scatter plot showing AcH3 levels (immunostaining) as a function of the nuclear elongation (aspect ratio of nuclei elliptical fitting) for both clones on flat surface versus grooves. As cells' nuclei become more elongated, AcH3 levels increase on the patterned substrate in clone 16, while no further acetylation is evoked in clone 3.