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. 2016 Mar 1;8:plw015. doi: 10.1093/aobpla/plw015

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Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Confirmation of inversion (3492 bp) between ndhC and rbcL in the genus Trillium. (A) Design of primer to amplify junction regions between atpB and rbcL regions. The positions of atpB and rbcL genes in LSC regions are drawn based on the sequence assembly results of T. tschonoskii, T. maculatum in this study (red text). *The data downloaded from the NCBI. The forward primer I1F contains the sequence in atpB region. The sequence of the reverse primer (I1R) is located in the rbcL gene. Polymerase chain reaction amplification of IGS between atpB and rbcL. Relationships of Parideae lineages followed the phylogenetic trees of S. C. Kim, J. S. Kim, W. C. Mark, F. F. Michael and J. H. Kim (unpublished data). (B) Primers were designed to amplify junction regions between trnV-UAC and rbcL regions. The positions of trnV-UAC and rbcL genes in LSC regions are drawn based on the sequence assembly results of T. tschonoskii, T. maculatum in this study (red text). *The data downloaded from the NCBI. The forward primer I2R contains the sequence in trnV-UAC region. The sequence of the reverse primer I1R is located in the rbcL gene. Polymerase chain reaction amplification of IGS between trnV-UAC and rbcL. Relationships of Parideae lineages followed the phylogenetic trees of S. C. Kim, J. S. Kim, W. C. Mark, F. F. Michael and J. H. Kim (unpublished data).