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Intramembranous and endochondral ossification. A, Postnatal day 1 skull vault stained with alizarin red (bone) and alcian blue (cartilage) showing sutures and fontanelles. B, Schematic representation of intramembranous bone formation at a skull suture. C, Proximal tibial section at P21 growth plate stained with alcian blue (cartilage) and Van Gieson (bone matrix, red). D, Schematic representation of the growth plate.
