Table 3.
Overview of species identification obtained with PCR and sequencing methods and comparison to the results of preoperative blood cultures (preBC).
| PCR positive n = 40, species identification n = 33∗ | |||||
|---|---|---|---|---|---|
| Concordance n = 9 (PCR/preBC∗∗) | n | Discordance n = 14 (on genus and/or species level) | Additional information n = 10 (no preBC data available) | n | |
| PCR | Result of preBC∗∗ | ||||
| Enterococcus faecalis | 5 | Enterococcus faecalis | Streptococcus gordonii | Enterococcus faecalis | 1 |
| Staphylococcus aureus | 1 | Enterococcus faecium | Enterococcus faecalis | Staphylococcus aureus | 2 |
| Streptococcus agalactiae | 1 | Staphylococcus aureus | Staphylococcus auricularis | Staphylococcus epidermidis | 1 |
| Aerococcus urinae | 1 | Staphylococcus epidermidis | Streptococcus oralis | Staphylococcus lugdunensis | 1 |
| Gemella haemolysans | 1 | Streptococcus gallolyticus subsp. pasteurianus | Streptococcus alactolyticus | Streptococcus mitis | 1 |
| Streptococcus gordonii | Streptococcus pneumoniae | Streptococcus pneumoniae | 1 | ||
| Streptococcus gordonii | Streptococcus viridans | Streptococcus sanguinis | 1 | ||
| Streptococcus mitis | Streptococcus intermedius | Anoxybacillus spp. | 1 | ||
| Bartonella quintana | Staphylococcus epidermidis | Propionibacterium acnes | 1 | ||
| Granulicatella adiacens | Streptococcus viridans | ||||
| Streptococcus oralis | Streptococcus viridans | ||||
| Enterococcus faecium | Gram-positive cocci | ||||
| Staphylococcus epidermidis | Gram-positive cocci | ||||
| Propionibacterium acnes | Negative preBC | ||||
∗In 7 patients with positive PCR amplicon, direct sequencing failed due to a bacterial mixture n = 4 or low DNA concentrations n = 3.
∗∗Results of preBC were taken and analyzed in hospitals of primary care according to local standard microbiological laboratory procedures.