Table 2.
Sample collection sites, phenotypes of donor and transconjugants, conjugation efficiency, resistance genes, and associated plasmid replicons.
| District | Resistance phenotypes of donors | Abx used for selection | Resistance genes in transconjugants | Conjugation efficiency | Replicon type in transconjugants | Resistance phenotypes of transconjugants |
|---|---|---|---|---|---|---|
| Arusha urban | StrSulTri | Str | ND | — | I1 | Str |
| StrTri | Str | ND | — | FIC | Str | |
|
| ||||||
| Moshi urban | AmpStrSulTetTri | Tet | tet(B), TEM-1 | 5.8 × 10−3 | ND | AmpStrSulTetTri |
| AmpStrSulTetTri | Amp | tet(A) | 3.83 × 10−3 | P, FIIA | AmpStrSulTetTri | |
| AmpStrSulTet | Amp | tet(A) | 9.88 × 10−1 | ND | AmpStrSulTet | |
| SulTetTri | Tet | tet(A) | 9.93 × 10−1 | ND | SulTetTri | |
| StrTetTri | Tet | tet(B) | 2.00 × 10−7 | ND | StrTetTri | |
| AmpStr | Amp | ND | 2.7 × 10−4 | FIIA | Amp | |
|
| ||||||
| Moshi rural | AmpStrSulTetTri | Amp | tet(A), TEM-1 | 8.33 × 10−6 | A/C, P, FIB | AmpStrSulTetTri |
| AmpSulTetTri | Tet | tet(A) | 7.86 × 10−2 | FIA, FIB | AmpSulTetTri | |
| AmpStrSulTetTri | Tet | tet(A) | 2.05 × 10−7 | A/C | AmpStrSulTetTri | |
|
| ||||||
| Hai rural | AmpStrSulTetTri | Amp | ND | 7.49 × 10−3 | ND | Amp |
| AmpStrSulTet | Amp | ND | — | ND | Amp | |
|
| ||||||
| Simanjiro rural | AmpCeftazKanStrSulTetTri | Amp | bla CTX-M-15 | 6.5 × 10−2 | N, FIB, FIA | AmpStrSulTetTri |
| AmpCeftazChloStrSulTetTri | Amp | bla CTX-M-15 | — | N, H12 | AmpCeftazStrSul | |
| AmpStrTetTri | Tet | tet(B) | 1.08 × 10−4 | ND | AmpStrSulTet | |
| AmpSulTetTri | Amp | ND | 6.25 × 10−4 | ND | AmpSul | |
| TetTri | Tet | ND | — | ND | Tet | |
|
| ||||||
| Monduli rural | AmpCeftazChloKanStrSulTetTri | Tet | tet(A), bla TEM-1 | 9.26 × 10−2 | H12, K/B | AmpCeftazChloKanStrSulTetTri |
| AmpStrSulTetTri | Amp | tet(A) | 3.19 × 10−4 | ND | AmpSulTet | |
| AmpStrSulTet | Amp | ND | 1.07 × 10−2 | I1, FIB | AmpSul | |
| AmpSulTet | Amp | ND | 7.91 × 10−3 | B/O | AmpSulTet | |
| AmpSul | Amp | ND | 2.45 × 10−2 | ND | AmpSul | |
|
| ||||||
| Longido rural | AmpStrSulTetTri | Amp | ND | 5.31 × 10−3 | B/O, FIC | AmpSul |
| AmpStrSulTri | Amp | ND | 8.93 × 10−4 | FIC | AmpSul | |
| AmpSulTetTri | Tet | ND | 8.99 × 10−5 | FIC, A/C, FIIA | AmpSulTet | |
| AmpStrTet | Amp | ND | 1.33 × 10−4 | I1, K/B | AmpStrTet | |
|
| ||||||
| Arumeru periurban | AmpChloKanSulTetTri | Amp | tet(B), bla TEM-1 | 8.95 × 10−2 | I1, FIB, FIA, K/B | AmpKanStrSulTetTri |
| AmpChloSulTetTri | Amp | ND | 1.66 × 10−2 | ND | AmpSul | |
| AmpStrSulTetTri | Amp | ND | 5 × 10−3 | ND | Amp | |
| AmpSulTetTri | Amp | bla TEM-1 | 8.33 × 10−1 | ND | AmpSulTetTri | |
Abx, antibiotic; Amp, ampicillin; Ceftaz, ceftazidime; Cip, ciprofloxacin; Chlo, chloramphenicol; Kan, kanamycin; Str, streptomycin; Sul, sulfamethoxazole; Tet, tetracycline; Trm, trimethoprim; ND, not detected.