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. 2016 Mar 20;4(5):e12722. doi: 10.14814/phy2.12722

Figure 6.

Figure 6

Effects of siRNA‐mediated knockdown of PHD1, PHD2, and PHD3 in 832/13 cells. (A) Effects of three siRNA duplexes targeted against PHD1 (PHD1_1, PHD1_2, PHD1_3) on PHD1 gene expression. (B) The effects of the PHD1 siRNA duplexes on GSIS (n = 8–10). (C) Effects of three siRNA duplexes targeted against PHD2 (PHD2_1, PHD2_2, PHD2_3) on PHD2 gene expression. (D) The effects of the PHD2 siRNA duplexes on GSIS (n = 8–10). (E) Effects of three siRNA duplexes targeted against PHD3 (PHD3_1, PHD3_2, PHD3_3) on PHD3 gene expression. (F) The effects of the PHD3 siRNA duplexes on GSIS (n = 8–10). PHD gene expression was corrected for by an internal control gene (cyclophilin) and then expressed as percent of control, *P < 0.05, **P < 0.01, ***P < 0.001 for NT cells versus siRNA‐treated cells. White and black bars represent secretion at 2 and 10 mmol/L glucose, respectively. siCtrl is a siRNA duplex with no known target. *P < 0.05, **P < 0.01, ***P < 0.001 for 10 mmol/L glucose‐treated NT cells versus 10 mmol/L glucose siRNA‐treated cells.