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. 2016 Mar 20;4(5):e12722. doi: 10.14814/phy2.12722

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effects of EDHB and siRNA‐mediated knockdown of PHD3 on gene and protein expression in 832/13 cells. HIF1α (A) mRNA expression and (B) protein expression in response to EDHB. mRNA expression of ARNT/HIF1β in cells treated with (C) EDHB and (E) a siRNA against PHD3. HIF1α mRNA expression (D) in cells treated with a siRNA against PHD3 (n = 6–8). Gene expression was corrected for by an internal control gene (cyclophilin) and then expressed as percent of control. γ tubulin served as a protein loading control.**P < 0.01, ***P < 0.001.