Figure 2.

Analysis of the expression of lung-derived cytokines following sublingual immunotherapy. a-c, The relative mRNA expressions of IL-33 (a), TSLP (b), and IL-25 (c) from lung tissues of the respective treated groups were evaluated by CYBR green-based real-time PCR (n = 5 to 8 mice per group). Expression level was normalized to mouse HPRT mRNA. d, Western blot analysis of above-mentioned cytokines. e-i, The relative protein band intensity of full-length IL-33 (e), cleaved IL-33 (f), TSLP (h), and IL-25 (i). g, Full-length to cleaved IL-33 ratio. Expression level was normalized to mouse beta-actin protein. Data are expressed as mean ± SEM. ns, not significant. * P<0.05, ** P<0.01, and ***P<0.001