Figure 5. Thermal stability of LtrK and LtrR assessed by DSC.

(a) Irreversible thermal unfolding of LtrK before baseline correction. Two melts are shown using a scan rate of 1 °C min⁻¹. (b) Irreversible thermal unfolding of LtrK after baseline correction. The apparent T_m for LtrK calculated from DSC thermograms was scan rate dependent. (c) Reversible thermal unfolding of LtrR before baseline correction. Two successive melts are shown using a scan rate of 1 °C min⁻¹. (d) Reversible thermal unfolding of LtrR after baseline correction. The T_m calculated for LtrR from DSC thermograms was scan rate independent.