Table 1. Sequences used to generate the three synthesized HCCR-targeting shRNAs.

No.	Targeting sequence
shRNA-1	5′-gatccccACAGATCTGTGCACCAAGAttcaagagaTCTTGGTGCACAGATCTGTttttt-3′
	3′-gggTGTCTAGACACGTGGTTCTaagttctctAGAACCACGTGTCTAGACaaaaatcga-5′
shRNA-2	5′-gatccccTAAGATGTGAGAAGCATGGttcaagagaCCATGCTTCTCACATCTTAttttt-3′
	3′-gggATTCTACACTCTTCGTACCaagttctctGGTACGAAGAGTGTAGAATaaaaatcga-5′
shRNA-3	5′-gatccccTTGTGCAGCAAGAGAGACAttcaagagaTGTCTCTCTTGCTGCACAAttttt-3′
	3′-gggAACACGTCGTTCTCTCTGTaagttctctACAGAGAGAACGACGTGTTaaaaatcga-5′

All of these sequences were sliced by the Bgl II and Hind III enzymes and inserted into pSuper vectors.