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. 2016 Apr 7;6:24043. doi: 10.1038/srep24043

Figure 3. Sulfated coumarins as inhibitors of thrombin.

Figure 3

(A) Dose-response profiles of selected sulfated coumarins (and non-sulfated 3g) against thrombin in 20 mM Tris-HCl, 100 mM NaCl, 2.5 mM CaCl2, 0.1% PEG 8000, pH 7.4 at 25 °C. (B) Spectrofluorometric measurement of the affinity of 3g for thrombin labeled at the active site using fluorescein-FPRCK (λEX = 490 nm, λEM = 525 nm). Solid lines represent nonlinear regression fitting using quadratic equation 4 to calculate the KD of binding. (C) Dose-response profiles of 3g against factor Xa and factor XIa with comparison against thrombin. Also shown is the profile for 3g inhibition of thrombin in the presence of 100 nM antithrombin. Solid lines represent sigmoidal dose-response fits using equation 1 to calculate IC50, ∆Y and HS.