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. 2016 Apr 7;6:23769. doi: 10.1038/srep23769

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Copyright © 2016, Macmillan Publishers Limited

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(a) The swimming motility of ΔXOC_2335 and ΔXOC_2393 was significantly reduced compared with that of the wild-type and other gene-deletion strains. The complementation strains ΔXOC_2335(2335) and ΔXOC_2393(2393) with plasmid-borne full-length genes restored the swimming motility nearly to or completely to the wild-type level, respectively. (b) The double-gene deletion mutant ΔXOC_2335/XOC_2393 exhibited a similar swimming ability to the ΔXOC_2335 mutant. The swimming motility of different Xoc strains was evaluated on semisolid plates with 0.2% noble agar after incubating at 28 °C for 4 d. The ratios of colony diameter of the mutant strains to the wild type were shown. Bars are means ± standard error (SE). The letters (a–d) indicate significant difference (P < 0.05) by Duncan’s multiple range test.