Skip to main content
. 2016 Apr 7;6:23769. doi: 10.1038/srep23769

Figure 4. Point mutations in the GGDEF or EAL motifs of XOC_2335 abolish its function in the regulation of swimming motility.

Figure 4

(a) The gene variant constructs with point mutations encoding XOC_2335GADEF-GAAAF-HA or XOC_2335EAL-EAA-HA variant proteins did not complement the swimming motility of ΔXOC_2335 while the XOC_2335-HA construct partially restores the swimming motility of ΔXOC_2335. The colony diameters of different Xoc strains cultured on semisolid plates with 0.2% noble agar were shown. WT, the wild-type strain. (b) Expression of HA-tagged XOC_2335 and its variants XOC_2335GADEF-GAAAF or XOC_2335EAL-EAA in Xoc was detected by western blot analysis with an anti-HA antibody. EV indicates the wild-type strain transformed with the empty vector pVSP61 as negative control. The letters (a–c) indicate significant difference (P < 0.05) by Duncan’s multiple range test.