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. 2016 Apr 7;6:23769. doi: 10.1038/srep23769

Figure 7. Measurement of intracellular c-di-GMP levels in wild-type or mutant Xoc strains.

Figure 7

Assays were performed as described in the Methods. The c-di-GMP concentrations were quantified in the wild type (WT), ΔrpfG, ΔXOC_2335, ΔXOC_2393, ΔXOC_2335/2393 and ΔXOC_4190 mutants. An increased c-di-GMP concentration was revealed in ΔrpfG, ΔXOC_2335/2393, ΔXOC_2335, and ΔXOC_2393 in comparison with the wild-type strain. The c-di-GMP level in ΔXOC_4190 was similar to that in the wild-type strain. Data are presented as means ± SE. The letters (a–d) indicate significant difference (P < 0.05) by Duncan’s multiple range test.