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. 2016 Apr 7;6:23914. doi: 10.1038/srep23914

Figure 2. 82-kDa ChAT has altered genome association after exposure of cells to Aβ.

Figure 2

(A) ChIP-seq was performed for SH-SY5Y cells stably expressing 82-kDa ChAT treated with either vehicle or 100 nM oligomeric Aβ1–42 for 4 h. Genomic features identified for 82-kDa ChAT. Both treatment groups had over 50% of the peaks at intergenic regions, but Aβ1–42 treatment increased association with introns, promoters and exons. Average peak length for 82-kDa ChAT after either vehicle or Aβ1–42 -exposure was also tested. Treatment with Aβ1–42 significantly decreased the 82-kDa ChAT peak length. ***p < 0.001 (Student’s t-test). (B) Top DREME motif hits for 82-kDa ChAT after vehicle or Aβ1–42 -exposure of cells. The top motifs for both treatments revealed a TC2-3AT motif, while Aβ1–42 -treatment also revealed a known SATB1 binding motif (boxed region). (C) Example ChIP-seq tracks for 82-kDa ChAT after vehicle or Aβ1–42 -exposure of cells for regions of GAB2 and MAGI2 genes. Peaks are highlighted in blue for Aβ1–42 peaks and red for vehicle peaks. H3K27Ac is overlaid to show active transcription initiation sites.