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. 2016 Apr 7;6:24171. doi: 10.1038/srep24171

Figure 4. Defective implantation in Sox17 heterozygous mutant females.

Figure 4

(a,b) Blue dye staining showing implantation sites in WT (a) and Sox17+/GFP (b) uteri at DOP 5 after natural mating. Arrows and arrowheads show the ovaries and implantation sites, respectively. (b’) Unimplanted blastocysts recovered from Sox17+/GFP uterus by flushing. (c) Number of implantation site in females of the indicated genotype at DOP 5 after natural mating. Dots and bars show sample and mean values, respectively. (d–g) Blue dye staining showing implantation sites in WT (d,f) and Sox17+/GFP (e,g) uteri at DOP 7 after embryo transfer (ET). WT embryos were transferred either to the oviduct (d,e) or the uterus (f,g). (h,i) Implantation rates at DOP 6 or 7 in embryo-transferred females of the indicated genotype. WT embryos were transferred via the oviduct (h) or in the uterus (i), except for WT*, to which approximately a 1:1 mixture of WT and Sox17+/GFP embryos were transferred. *p < 0.05; **p < 0.01; ***p < 0.001 as per one-way analysis of variance Dunnett’s multiple comparisons test (c,h) and t-tests (i).