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. 2016 Apr 7;6:23968. doi: 10.1038/srep23968

Figure 6. Quercetin inhibits mTOR and β-catenin but not Smad signaling activation stimulated by TGFβ1 in NRK-49F cells.

Figure 6

NRK-49F cells were pretreated with quercetin (20 μM) for 30 mins, followed by TGFβ1 (2 ng/ml) treatment and harvested at various time points as indicated. (A) Western blot analyses demonstrating that quercetin could reduce the abundance of p-mTOR (Ser2448), p-p70 S6K (T421/S424), p-S6 (S235/236), p-Akt (Ser473), p-β-catenin (Ser675) except p-Smad3 (Ser423/425) and p-Smad1/5/9. The gels were run under the same experimental conditions. (B) Representative micrographs of immunofluorescent staining of p-S6 (Ser235/236), p-Akt (Ser473), β-catenin and p-Smad3 (Ser423/425) further confirmed the results of western blotting assay. White arrows indicate the cells with β-catenin nuclear translocation.