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. 2016 Apr 7;6:23975. doi: 10.1038/srep23975

Figure 5. Metformin prevents cisplatin-induced cell apoptosis in cultured NK-52E cells.

Figure 5

(A) The representative graphs showing the results of FACS analysis. NRK-52E cells treated with cisplatin w/o metformin were stained with annexin-V-FITC (20 mg/ml) and PI (20 mg/l) to identify cell apoptosis. (B) The graph showing the cell apoptosis among different groups. *P < 0.05 compared to control (n = 4); #P < 0.05 compared to cells treated with cisplatin alone (n = 4). (C) Representative micrographs showing TUNEL staining among different groups as indicated. (D) The graph showing the quantitative determination of TUNEL staining positive cells among different groups. Data are presented as the percentage of the TUNEL staining positive cells. *P < 0.05 compared to vehicle control (n = 3); #P < 0.05 compared to mice treated with cisplatin alone (n = 3). (E) Western blot assay showing the abundance of cleaved caspase 3 among different groups. The gels were run under the same experimental conditions.