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. 2016 Apr 7;6:24015. doi: 10.1038/srep24015

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HPLC analysis of the products of dehydration and hydration of 3HB-CoA (A) and crotonyl-CoA (B), respectively, as catalyzed by PhaJ1. (C) Evaluation of the stereospecificity of PhaJ1. The hydration of crotonyl-CoA as catalyzed by PhaJ1 coupled with crude PHA synthase located on nPHA granules that were isolated from H. mediterranei EPSΔphaJ1 was spectrophotometrically monitored at 412 nm.