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. 2016 Apr 7;6:24015. doi: 10.1038/srep24015

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Copyright © 2016, Macmillan Publishers Limited

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(A) The inhibitors of β-oxidation enzyme inhibited PHA mobilization in H. mediterranei. EPS, H. mediterranei EPS; Inhibitor, acrylic acid (11 mM) plus thioglycolic acid (1.2 mM). (B) qRT-PCR assay for analysis of the fold change of gene expression when H. mediterranei EPS cultured in fresh PAC medium without (−C) vs with (+C) glucose. Genes HFX_1509, HFX_2830, HFX_4016, and HFX_6355 encoding the 3-hydroxyacyl-CoA dehydrogenase in β-oxidation cycle were chosen. The fold change of gene expression at 1 h, 5 h, and 10 h was analyzed. The fold change of expression of each gene was calculated by normalization to the expression of inner control 7S RNA.