Figure 6. Effect of SIRT3 and SITR5 on ureagenesis in periportal hepatocytes.

(A) SIRT3 expression in periportal hepatocytes; (B,D) Acetylated OTC and CPS1 in control and SIRT3 knockdown hepatocytes; (C) Urea in hepatocytes cultured media; (E) Activities of OTC and CPS1 in hepatocytes; (F) Expression of SIRT5 in periportal hepatocytes; (G,I) Acetylated OTC and CPS1 in control and SIRT5 knockdown hepatocytes; (H) Urea in hepatocytes cultured media. (J) Activities of OTC and CPS1 in hepatocytes; (K) Viability of gene knockdown hepatocytes as determined by leakage of cytosolic lactate dehydrogenase to the culture media; (L) Activity of mitochondrial succinate dehydrogenase (respiratory chain complex II), assayed by MTT; (M) Levels of hepatocyte ATP 24-hours afer transfection: SIRT3/5(+) Glu(−) were the control group. Glu(+) and Glu(−) represent with- and without glucagon in medium, respectively. SIRT3(−) and SIRT3/5(+) represent hepatocytes transfected with si SIRT3/5 and control siRNA, respectively. Data are expressed as means and standard errors of each different experiment (n = 4 for each group). *P < 0.05 from control group, **P < 0.01 from control group.