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. 2016 Apr 6;7:11190. doi: 10.1038/ncomms11190

Figure 4. ABT-737 eliminates senescent cells from lung of irradiated mice.

Figure 4

(a) Seven days post irradiation, mice were treated with ABT-737 (n=5) or vehicle (n=5) for 2 consecutive days. Lungs were dissected 1 day thereafter, sectioned and the sections were stained for SA-β-Gal. Scale bar, 10 μm. (b) Quantification of SA-β-Gal staining in the bronchioles area of vehicle- or ABT-737-treated mice. Data were presented as means±s.e.m. from five mice in each group. (c) One day following vehicle or ABT-737 treatment, lung were dissociated, stained for γH2AX and analysed by FACS. Percentage of γH2AX-positive cells was quantified from this analysis. Data were presented as means±s.e.m. from five mice in each group. (d) Western blot analysis of Bcl-w, p53 and p21 in the mice described in a. Two mice represent each condition, each line represents a mouse. (e) Lung sections from unirradiated mice and the mice described in a were stained for Bcl-xL, p53 and cleaved caspase-3, a marker of apoptosis. Scale bar, 10 μm. Data in b,c were analysed using Student's t-test. *P<0.05, ***P<0.0005.