Fig. 7.

Effect of HHEX overexpression on the capacity of NB4 cells to express VEGF-A and to differentiate in response to ATRA. NB4 cells have been transfected with a control empty vector (pTWEEN) or with this vector containing the human HHEX gene (pHHEX) and then a analyzed for HHEX expression by Western blotting (top panel, left) and for VEGF-A RNA expression by realtime-PCR (top panel, right); b pTWEEN NB4 cells and pHHEX NB4 cells have been grown in the absence or in the presence of 1 μM ATRA and evaluated at different times for c the number of viable cells (middle panels) and for d the expression of CD11b, CD15, CD18, and CD54 membrane antigens (low panels). The results of the three experiments ± SEM are reported in the figure. For NB4 cells grown in the absence of ATRA, the expression level of CD18 and CD54 antigens between pTWEEN and pHHEX cells was significant (p ≤ 0.05). For NB4 cells grown in the presence of ATRA, the difference of CD15 (p ≤ 0.05) and CD11b, CD18, and CD54 (p ≤ 0.01) between pTWEEN and pHHEX cells was significant