Figure 1.

TGase 2 and HDM2 regulate p53 stability in an independent manner. ACHN (a and b) and CAKI-1 (c and d) cells were transfected with siRNA targeting TGM2 (a and c) or HDM2 (b and d) for 48 h; then the cells were treated with chloroquine (CQ, 50 μM) or MG132 (10 μM), or left untreated, for 6 h under starvation conditions before harvesting. Whole-cell lysates were subjected to immunoblotting with the indicated antibodies. ACHN (e and f) and CAKI-1 (g and h) cells were treated with siRNA for TGM2 or HDM2, and stained with TUNEL and DAPI (e and g). Scale bar, 20 μm. The percentage of TUNEL-positive cells was counted (f and h). The western blots are representative of three independent experiments. **P<0.01, ***P<0.001