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. 2016 Mar 3;7(3):e2130. doi: 10.1038/cddis.2016.26

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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LINC01225 promoted cell proliferation and invasion in vitro. (a) Edu assay confirmed the functional role of LINC01225 in cell proliferation (× 200). (b) The results of Edu assay. Decreased level of LINC01225 reduced the proliferation of SMCC7721 and MHCC97H after 24 h, compared with the control cells; overexpression of LINC01225 in LINC01225 knockdown cells restored and enhanced cell proliferation after 24 h. The integral optical density (IOD) values of cells treated with control plasmids were normalized to 100%. (c) Upper panels: CCK8 assay showed that LINC01225 stable knockdown inhibited growth of SMCC7721 and MHCC97H cells; cell growth was restored and enhanced in LINC01225 knockdown cells treated with Lv-Rescue plasmid. Lower panel: Absorbance at 450 nm was presented as the mean±S.E.M. Absorbance at 450 nm of cells treated with control plasmids was normalized to 100%. Data were collected and provided at 24 h after cultivation. (d) Left panels: Morphology of invasive SMCC7721 and MHCC97H cells after stable transfection with negative control, LINC01225 shRNA or Lv-Rescue plasmid (× 400). Right panel: The number of cells treated with control plasmid was normalized to 100%, and data are presented as the mean±S.E.M., based on at least three independent experiments (*P<0.05, **P<0.01, ***P<0.01)