Figure 2.

PGC-1α is a direct target of miR-485-3p and miR-485-5p. (a) Six predicted target genes (PGC-1α, SENP1, TCF7, Spred2, KLF6 and Cul3) of miR-485-3p and miR-485-5p by Targetscan, microRNA.org and DIANA TOOLS. (b) The expression levels of predicted target genes in MCF-7 and MDA-MB-231 cells with transfection of miR-control, miR-485-3p or miR-485-5p. β-Actin was used as an internal control. *P<0.05 by one-way ANOVA compared with miR-control-transfected cells. (c) Western blot analysis of PGC-1α in MCF-7 and MDA-MB-231 cells transfected with miR-control, miR-485-3p or miR-485-5p. β-Actin was used as loading control. *P<0.05 by one-way ANOVA compared with miR-control-transfected cells. (d) Schematic representation of the wild-type PGC-1α 3′-UTR (WT) showing putative miR-485-3p and miR-485-5p target sites. (e) Mutations of predicted binding sites of miR-485-3p (MT1) or miR-485-5p (MT2) in the PGC-1α 3′-UTR. (f) Relative luciferase activity in HEK293T cells co-transfected with WT or MT reporter plasmid and miR-control, miR-485-3p or miR-485-5p. *P<0.05 by t-test compared with miR-control-transfected cells