Table 1. Summary of necroptosis in different AKI models in vitro.
| Year | Cell line/type | Induction | Therapeutic intervention | Effect of zVAD | Reference |
|---|---|---|---|---|---|
| 2012 | Mice tubular cell line TKPTS, mice glomerular endothelial cell line glENDp54 | TNF-α/cycloheximide in the presence of zVAD | Nec-1; Rip1 siRNA; Rip3 siRNA | NA | 47 |
| 2012 | Human proximal tubular cell HK-2 | Cisplatin | Nec-1; Nec-1+zVAD | Protective | 54 |
| 2012 | Rat tubular cell line NRK-52E | CsA | Nec-1; Rip3 siRNA | NA | 57 |
| 2013 | Rat tubular cell line NRK-52E | TNF-α/antimycin A | Nec-1 | NA | 49 |
| 2013 | Mice renal proximal tubular cells | TNF-α/TWEAK/IFN-γ | Nec-1 | Harmful | 51 |
| 2014 | Human proximal tubular cell HK-2 | TNF-α/antimycin A in the presence of zVAD | Nec-1 | NA | 50 |
| 2015 | Mice renal proximal tubular cells | Cisplatin | Nec-1; Rip3-KO; Mlkl-KO; Rip1 shRNA | No effect | 55 |
Abbreviations: CsA, cyclosporin A; IFN, interferon; TNF, tumor necrosis factor; TWEAK, TNF-related weak inducer of apoptosis.