Figure 2.

MiR-125a-5p is reduced after HOTAIR knockdown. (a) Heat map of genes with significant induction (red) or repression (green) at sixth hour after 50 nM siHOT1 transfection into HCT116 cells (expressed as a ratio to HCT116 cells transfected with siNC, fold change >2, P-value <0.05, data are log₂ transformed). (b) Quantitative real-time PCR validation of a representative panel of genes detected down-regulation by RNA-seq. Raw Ct values were normalized to U6 RNA. Bars represent mean±S.E.M. from three independent experiments. (c) FCM detected the rescue effect of miRNAs on apoptosis induced by HOTAIR knockdown. (d) Inhibition of miR-125a-5p caused apoptosis. (e) miR-125a-5p declined after RNAi of EZH2 and LSD1. Bars represent the mean±S.E.M. from three independent experiments. *P<0.05, **P<0.01 and ***P<0.001 by Student's t-test. All experiments were performed in three biological repeats