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. 2016 Mar 31;7(3):e2168. doi: 10.1038/cddis.2016.50

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Autophagy is required for the fusion of myocytes into multinucleated myotubes. WB analysis of MHC (200 kDa), desmin (53 kDa) and LC3-I (18 kDa) and II (16 kDa) in whole-cell extracts from muscle cells allowed to terminally differentiate and fuse in multinucleated myotubes (a) or to reach the status of fully differentiated myocytes by preventing cell contact (b). A mix of ammonium chloride and leupeptin (NH₄Cl/Leu, 20 mM/100 μM) was added to the medium 2 h before cell harvest as indicated. (a and b) Bottom: fold changes of the indicated proteins relative to the loading control (l.c.) were determined by densitometric scanning. One representative experiment (out of two) is shown