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. 2016 Mar 17;7(3):e2146. doi: 10.1038/cddis.2016.52

Figure 4.

Figure 4

mCD40L-induced TNF-α does not contribute to CD40L-induced cell death. (a) EJ cells were infected with 100 MOI of either RAdMock (AdM) or RAdnCD40L (AdnL) or left uninfected as a negative control for 24 h, RNA was extracted utilising the EZ-RNA total isolation kit and the cDNA was prepared by reverse transcription. The expression of TNF-α was examined by qRT-PCR technique. Results are mean of triplicate samples ±S.D. (b) EJ cells were infected with 100 MOI of either RAdMock (AdM) or RAdnCD40L (AdnL) or left uninfected as a negative control and plated at a density of 6000/100 μl/well in 96-well microplate. AdnL-infected cells were either treated with 1, 3 or 5 μg/ml of TNF-α monoclonal neutralising antibody or left untreated as a control for 28 h. Cell viability was then assessed using the WST-1 assay. Results are mean of triplicate samples ±S.D.