Figure 5.

TBPT inhibited tubulin polymerization and disrupted microtubule formation. (a) The rate of the tubulin polymerization process was monitored via fluorescence in the presence of TBPT or colchicine (CH) at the indicated concentration in a cell-free system. The fluorescence was monitored every 2 min for 50 min. (b) Dose-dependent tubulin polymerization was observed. N=2. (c) After the cells were treated with DMSO, TBPT or CH for 24 h, their cellular microtubules were visualized via green fluorescence using a fluorescent secondary antibody binding specifically to anti-α-tubulin antibody. The scale bars represent 10 μm. (d) The molecular docking study of TBPT to the CH-binding site of tubulin