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. 2016 Mar 24;7(3):e2153. doi: 10.1038/cddis.2016.58

Figure 4.

Figure 4

ARTD1 regulates Blimp1 expression through epigenetic mechanisms. (a) Western blot analysis of WT ARTD1 and ARTD1D214N expression in BMM (Artd1D/D, Artd1D214N/D214N). (b) Western blot analysis of Blimp1 and PU.1 expression in BMM, pOC (d2) or OC (d3). Results are from the same gels, but the lanes were cut and pasted). (c) Analysis of global H3K4me3. (d) Analysis of global H3K27me3. (e) ChIP analysis of PU.1 binding to the Blimp1 promoter using IgG or PU.1 antibody for immunoprecipitation. (f) ChIP analysis of H3K4me3 at the Blimp1 promoter. Data are expressed as mean±S.D. *P<0.05, BMM versus pOC, and are representative of three independent experiments