Figure 1.
Anti-proliferative potential of GW627368X. (a) Dose-dependent inhibitory effect of GW627368X on growth of cervical cancer cell lines. Cervical cancer cell lines HeLa, SiHa, ME 180 and normal cell lines NIH/3T3, HaCat and HBL-100 were treated with various concentrations of GW627368X for 24 and 48 h. Points, average±S.D., representative of three independent experiments each performed in triplicates, P<0.05. (b) Cell cycle analysis. Indicated cell lines were treated slightly lower than IC50 (48 h) dose of the drug (9, 10 and 10 μM for Hela, SiHa and ME 180, respectively) in treatment groups and 0.1% DMSO in control for indicated time periods. (c and d) Live dead assay. (c) Micrographs representing cells stained with Calcein AM and Ethidium bromide homodimer after treating the cells for indicated time periods. Viable cell population appears green (calcein AM) whereas non-viable/dead cells appear as orange/red (ethidium bromide) in the fluorescent micrographs. (d) Graphical representation of percentages of live and dead cells represented as histogram, Values presented as mean±S.D.; *P<0.05, **P<0.01, ***P<0.001