Figure 3.
GW627368X impedes migration and invasion of cervical cancer cells. (a and b) Wound healing assay. Cells were allowed to grow in monolayers in six-well plates and the monolayer was wounded with a pipette tip. Cells were then treated with one third IC50 of GW627368X. The control cells were treated with 0.1% DMSO. (a) The wounds were observed and photographed till 48 h at regular intervals. (b) Graphical representation of wound size at different time points. (c and d) Boyden chamber assay. (c) Micrographs representing invasion of HeLa, SiHa and ME 180 cells following treatment with GW627368X. (d) Graph depicting percentage of cells invading the inserts of Boyden chamber coated with matrigel, representative of three different experiments each performed in triplicates, values presented as mean±S.D.; *P<0.05, **P<0.01, ***P<0.001