Figure 6.

Hindrance of EP4/EGFR cross-talk validated by exogenous overexpression of PGE2 and EGFR blockade. (a and b) Cells were stimulated with exogenous PGE2 (100 nM) and phosphorylation status of stimulated or unstimulated cells with or without drug treatment was studied by western blot. (a) Representative blots showing phosphorylation levels of EGFR in cervical cancer cell lines. (b) The relative levels of pEGFR expression was determined by densitometry. Data presented as mean±S.D., P<0.05, **P<0.01, ***P<0.001 of three independent experiments performed in triplicates. (c–h) Cells were stimulated with exogenous PGE2 (100 nM) and EP4, EGFR or both were blocked with GW627368X9 (9, 10 and 10 μM of drug for HeLa, SiHa and ME 180), EGFR mAb (20 ng/ml) or both and phosphorylation status of key downstream molecules were studied by western blot. (c, e and g) Representative blots showing activation levels of key regulator proteins in ME 180, HeLa and SiHa, respectively. (d, f and h) The relative levels of protein expression was determined by densitometry. Data presented as mean±S.D., P<0.05, **P<0.01, ***P<0.001 of three independent experiments performed in triplicates