Figure 7.

Validation of the effect of EP4 inhibition in cervical cancer using CJ-023423, a highly selective EP4 inhibitor as positive control. (a) Dose-dependent inhibitory effect of CJ-023423 on growth of cervical cancer cell line, ME 180. ME 180 cells were treated with various concentrations of GW627368X for 48 h. Points, average±S.D., representative of three independent experiments each performed in triplicates, P< 0.05. (b and c) ME 180 cells were treated with GW627368X (10 μM) and CJ-023423(7 μM) followed by stimulation with EGF. The relative phosphorylation levels of important signaling mediators were studied by western blot analysis. (b) Representative blots showing western blot analysis of key proteins. (c) The relative levels of phosphorylation of each protein was determined by densitometry. Data presented as mean±S.D., P<0.05, **P<0.01, ***P<0.001 of three independent experiments performed in triplicates