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. 2016 Mar 24;7(3):e2152. doi: 10.1038/cddis.2016.65

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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NHA1 and NHA2 were specifically expressed in the principal piece of sperm tail. (a-f) Immunofluorescence staining of NHA1 in mouse testis (a and b), cauda epididymis (c and d) and sperm from cauda epididymis (e). Note the intensive green signal at principal piece of sperm tail. NHA1 antibody staining in the presence of competing immunogen (f). The nuclei are counterstained with DAPI (blue). (g) Phylogenetic tree displays the relationship between the NHA1 and the other NHEs. The tree was generated with GeneBee aligning the predicted open reading frames of each NHE. (h-j) Immunofluorescence staining of NHA2 in mouse testis (h), cauda epididymis (i) and sperm from cauda epididymis (j). Scale bar in a, c, h and i, 100 μm. Scale bar in b and d, 50 μm. Scale bar in e, f and j, 20 μm