Figure 2.

Diagram illustrates our targeting strategy and the creation of Nha1 cKO mice. (a) In the targeted allele, a loxP site and a pgk-neo cassette flanked by two loxP sites are inserted into intron 3 and intron 4, respectively. Mice carrying the Nha1-targeted allele were crossed with the Cre transgenic mice to generate progeny carrying the Nha1Fx or Nha1△ allele. (b) PCR analysis detects the presence of 5' (PCR: P1–P2) and 3' (PCR: P3–P4) loxP sites for genotyping the wild-type (+/+), heterozygous (Fx/+) and homozygous (Fx/Fx) mice, and examines the deletion of exon 4 in the Nha1△/+ mice (PCR: P1–P4). (c) The knockout efficiency was confirmed by western blotting in testis and sperm samples. The protein level was normalized and plotted against β-tubulin. The data are expressed as the mean±S.D. **P<0.01