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. 2016 Mar 24;7(3):e2152. doi: 10.1038/cddis.2016.65

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Testis and sperm morphology of Nha1 cKO mice. (a-c) Testicular weight (a), epididymal weight (b) and sperm count (c) were examined. No overt abnormalities were found in the Nha1 cKO mice. Data are expressed as the means±S.D. (n=5). (d) Testicular and cauda epididymal sections stained with H&E and spermatozoa obtained form cauda epididymis. No overt morphological abnormalities were observed in the testis or spermatozoa of Nha1 cKO mice. (e) Sperm principal piece (P; upper panels) and midpiece (M; lower panels) were observed with TEM. No structural abnormalities were observed. Scale bar in d (the first two columns), 100 μm. Scale bar in d (third column), 10 μm. Scale bar in e, 200 nm