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. 2016 Mar 31;7(3):e2169. doi: 10.1038/cddis.2016.75

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Pdcd4 deficiency increases the expression levels of stem cell-related phenotypes on ADSCs. ADSCs were isolated from epididymal fat pads of WT and Pdcd4^−/− mice (n⩾8 per group) and expanded in DMEM containing 10% FBS and bFGF (5 ng/ml). (a) The morphology of WT and Pdcd4^−/− ADSCs was examined by light microscope. Scale bar=200 μm. (b) The expression of stem cell-related positive markers CD90 and CD105 and negative makers CD11b and CD11c was detected by flow cytometry. Data are representative of three independent experiments