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Validation of Bre null mutation in the skeletal muscles of BRE-KO mice. (A) PCR genotyping showing exon 3 of the Bre gene has been deleted in Bre−/− tissue. RT-qPCR (B) and Western blot (C) analysis also confirm the Bre−/− skeletal muscles do not express Bre mRNA and protein, respectively. GAPDH was used as internal control.
