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. 2016 Jan 6;5(2):100–111. doi: 10.1242/bio.012450

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 10. — RT-qPCR analysis revealed that BRE-KO satellite cells expressed a significantly higher level of CXCR4 mRNAs (40 folds higher) than BRE-WT satellite cells (B, *P<0.05). (C) There was no significant difference in HGF receptors (c-Met) expression. (D–E′) Surprisingly, immunofluorescent staining indicated that BRE-WT satellite cells (E) were more intensely stained for CXCR4 expression than BRE-KO satellite cells (D). (F) Western blot analyses of CXCR4 were performed before and after 1 h SDF-1α treatment. (G) Statistical analysis revealed that CXCR4 was significantly degraded in both BRE-KO and BRE-WT satellite cells after 1 h SDF-1α treatment (**P<0.01). However, CXCR4 degradation in BRE-KO cells was significantly more rapid than in BRE-WT cell (50% vs 20%, respectively). Data are presented as mean±s.e.m. Scale bars=20 μm.