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. 2016 Jan 6;5(2):100–111. doi: 10.1242/bio.012450

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 9. — (A-D) Time-lapse microscopy was used to record the migration of satellite cells in vitro. Individual cell trajectory plots indicated that BRE-KO (A) and BRE-WT (B) migrated in all directions and there were no significant differences in directionality between the two groups (C). By contrast, there was a significant reduction in the velocity of BRE-KO satellite cells' movement compared with BRE-WT (D, ***P<0.001). (E-G) DiI-labelled BRE-KO (red, E) and DiO-labelled BRE-WT (green, F) satellite cells were tested for their abilities to chemotactic response to SDF-1α. (K) Statistical analysis revealed that BRE-KO satellite cells were significantly less responsive to SDF-1α than BRE-WT cells (**P<0.001). By contrast, when HGF was used as the chemoattractant (H-J), there was no difference in chemotactic response and migration (L). Data are presented as mean±s.e.m. Scale bars=100 μm.