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. 2016 Jan 15;5(2):130–139. doi: 10.1242/bio.014415

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Lack of epithelial bending in Cdc42-deficient thyroid buds at E10.5. (A-H) Immunostaining of thyroid buds from control (A,C,E,G) and conditional knockout (cko; B,D,F,H) thyroid buds for E-cadherin (A,B) and ZO-1 (C,D), with nuclei counterstained with DAPI (E,H), merged in (G,H). (I) Tracing of the cell outlines at the point of epithelial bending in the thyroid bud from a control embryo. (J) Trace of the cell outlines in the central portion of the unbent epithelium of the thyroid bud from a conditional knockout embryo. Scale bar=100 μm.