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Early thyroid bud development is abnormal in Shroom3 deficient embryos. Immunofluorescently labeled cryosections through the developing thyroid of control and Shroom3Gt/Gt embryos at the indicated embryonic ages. Sections were labeled with phalloidin to detect F-actin (A-D,G,H), or immunostaining for NKX2-1 (E-H). Scale bars=100 µm.
