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EGFR steady-state kinetic parameters for Shc1 peptides
| Peptide | Sequence | Km (μM) | kcat (s−1) | kcat/Km (μM−1s−1) |
|---|---|---|---|---|
| Y-Y | PDHQYYNDAKKK | 1829 ± 172 | 0.060 ± 0.006 | 3.34E-5 |
| Y-pY | PDHQYpYNDAKKK | 488 ± 34 | 0.063 ± 0.008 | 1.30E-4 |
Kinetic parameters were determined by unweighted, nonlinear least squares analysis. Values represent means from three separate experiments ± s.e.m. The phosphorylated tyrosine is underlined.
