Fig. 5.

Markers of oxidative damage in cultured lung epithelial cells and protection by αKlotho protein. A549 cells were treated with 200 nM H₂O₂ for 3 h with different concentrations of recombinant αKlotho. A: DNA (8-OHdG). B: protein (protein carbonyl). C: lipid (8-isoprostane) oxidative damage. Average of 3 independent experiments (means ± SD). Statistical significance was evaluated by ANOVA. *P < 0.0001 vs. untreated cells, †P < 0.0001 vs. cells treated with 200 nmol/l H₂O₂ and 0 pM αKlotho, ‡P < 0.001 vs. cells treated with 200 nmol/l H₂O₂ and 25 pM αKlotho, §P < 0.05 vs. cells treated with 200 nmol/l H₂O₂ and 50 pM αKlotho, #P < 0.0001 vs. cells treated with 200 nmol/l H₂O₂ and 100 pM αKlotho.