Table 2.

Mitochondrial enzyme activity and intrinsic H₂O₂ emission

	Vehicle		MitoTEMPO
	Sham	CHF	Sham	CHF
COX activity, U/mg protein	708 ± 26	902 ± 52*	649 ± 30	907 ± 56*
CS activity, AU·min−1·mg protein−1	4.0 ± 0.2	3.9 ± 0.4	4.2 ± 0.5	4.2 ± 0.3
Mitochondria JH2O2, pmol·min−1·COX activity −1	34 ± 6	130 ± 11†	52 ± 12	43 ± 10
Mitochondria JH2O2, pmol·min−1·CS activity −1	6.4 ± 1.2	31 ± 5.6†	8.0 ± 2.3	8.8 ± 1.6

Values are means ± SE. COX, cytochrome c oxidase; CS, citrate synthase; AU, arbitrary units. Intrinsic H₂O₂ emission (JH₂O₂) calculated as mitochondria JH₂O₂ (Fig. 1) divided by COX or CS activities.

^*P < 0.05 vs. Sham within treatment.

^†P < 0.05 vs. all other groups.